400 MHz Nuclear Magnetic Resonance (NMR)

Nuclear Magnetic Resonance Spectroscopy is used to elucidate the chemical structure of a molecule and is used heavily in our Organic Chemistry laboratories.  Students usually take the Organic I and II labs in their sophomore year.

 

 

High Performance Liquid Chromatography (HPLC)

High Performance Liquid Chromatography is used to separate mixtures of chemical compounds.  HPLC utilizes different types of stationary phases and a liquid mobile phase that separate sample components based on their physical properties.  HPLC is used in many of the upper division laboratories as well as in undergraduate research.

 

 

Gas Chromatography (GC)

Gas Chromatography is used to separate and analyze compounds in a sample that can be turned into a vapor without being destroyed.  GC utilizes a capillary column which depends on the column's solid phase and dimensions. The difference in the chemical properties between different molecules in a mixture will separate the molecules as the sample travels the length of the column. GC is used heavily in the Organic, Analytical Chemistry, and upper division laboratories.

 

 

Gas Chromatography Mass Spectrometry (GC-MS)

GC- MS combines Gas Chromatography with a Mass Spectrometer to identify different substances.  The GC separates complex gaseous mixtures into individual compounds and the mass spectrometer is used to capture, ionize, accelerate, deflect, and detect the ionized molecules separately.  The GC-MS is used in the Organic and Analytical laboratories as well as in undergraduate research.

 

Fourier Transform Infrared Spectroscopy (FTIR)

FTIR is a technique that sends a beam of light through a sample and collect information on how much of the beam is absorbed by the sample at different frequencies of light.  This reveals information about the different components of the sample’s chemical structure.  FTIR is used heavily in the Organic and Advanced Inorganic laboratories.

 

Ultraviolet/Visible Spectroscopy (UV-Vis)

UV/Vis spectroscopy refers to absorption or reflectance spectroscopy in the ultraviolet and visible regions of the electromagnetic spectrum.  The absorption wavelengths and magnitude in these regions are used to characterize molecules.

 

 

Atomic Absorption Spectroscopy (AA)

Atomic absorption spectroscopy  is used for the qualitative and quantitative determination of chemical elements employing the absorption of optical radiation (light) by free atoms in the gaseous state.  AA is used by freshman in the Principles of Chemistry laboratory.  Students also use AA in the analytical chemistry, physical chemistry, and advanced inorganic laboratories.

 

Differential Scanning Calorimetry (DSC)

DSC’s monitors heat flow during phase changes induced by increasing the temperature.  DSC’s can be used to determine different physical and chemical properties of materials such as melting temperatures.  DSC is used in the Organic and Biochemistry laboratories.

 

 

Fluorescence Spectroscopy

Fluorescence Spectroscopy is a type of electromagnetic spectroscopy that analyzes the fluorescence of a sample. It involves using a beam of light to excite the electrons in molecules and cause them to emit light.  Fluorescence Spectroscopy is used in the Analytical, Biochemistry, and Physical Chemistry laboratories.

 

 

Polarimetry

A polarimeter is used to measure the angle of rotation caused by passing polarized light through a substance. Many molecules are optically active, and polarized light will rotate either to the left (counter-clockwise) or right (clockwise) when passed through these substances. The amount by which the light is rotated is known as the angle of rotation.

 

Ion Chromatography

Ion Chromatography is a process that allows the separation of ions and polar molecules based on their charge. It can be used for almost any kind of charged molecule and is commonly used in Analytical and Biochemistry.